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Genereux Research Group

Analytical Tools to Investigate Proteostasis

Publications

 

* represents co-first authors, ¥ undergraduate authors, § co-corresponding authors.
 

Articles
 

  1. Quanrud GM*, Abernathy M*, Lyu Z, Ying SC§, Genereux JC§. Hsp40 Affinity Profiling Reveals Protein Destabilization Profiles Following Cellular Manganese and Vanadate Exposure. ChemRxiv 2023.
  2. Lyu Z, Genereux JC. Quantitative Measurement of Transthyretin Mistargeting by Proximity Labeling and Parallel Reaction Monitoring. BioRxiv 2023, Front Chem Biol 2023, 2:1288188.
  3. Espinoza MF*, Nguyen KK*, Sycks MM, Lyu Z, Quanrud GM, Montoya MR, Genereux JC. Heat Shock Protein Hspa13 Regulates Endoplasmic Reticulum and Cytosolic Proteostasis Through Modulation of Protein Translocation. Biorxiv 2022, J Biol Chem 2022, 298, 102597. 
  4. Quanrud GM, Lyu Z, Balamurugan S¥, Canizal C¥, Wu H-T, Genereux JC. Cellular Exposure to Chloroacetanilide Herbicides Induces Distinct Protein Destabilization Profiles. ChemRxiv 2022, ACS Chem Biol, in press.
  5. Mezgarzadeh JS, Romine IC, Smith-Cohen EM, Grandjean JMD, Kelly JW, Genereux JC, Wiseman RL. ATF6 Activation Reduces Amyloidogenic Transthyretin Secretion Through Increased Interactions with Endoplasmic Reticulum Proteostasis Factors. Biorxiv 2022, Cells, 11, 1661. https://www.mdpi.com/2073-4409/11/10/1661
  6. Montoya MR, Quanrud GM, Mei L, Montaño JL¥, Genereux JC. Evaluating Client Protein Recovery by the Hsp40s DNAJB8 and DNAJB1 with AP-MS. Biorxiv 2022.
  7. Clery R, Armendi A¥, Franco V¥, Furrer S, Genereux JC, Kahn T, Koshiro K¥. Chemical Diversity of Citrus Leaf Essential Oils. Chem Biodiv 2022, 19, e202100963. doi.org/10.1002/cbdv.202100963
  8. Lyu Z*, Sycks MM*, Espinoza MF, Nguyen KK, Montoya MR, Galapate CM¥, Mei L, Genereux JC. Monitoring Protein Import into the Endoplasmic Reticulum in Living Cells with Proximity Labeling. Biorxiv 2021, ACS Chem Biol 2022, accepted.
  9. Quanrud GM, Montoya MR, Mei L, Awad MR¥, Genereux JC. Hsp40 Affinity to Identify Proteins Destabilized by Cellular Toxicant Exposure. Biorxiv 2021, Anal Chem 2021, 93, 16940-16946. https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c04230 
  10. Mei L*, Montoya MR*, Quanrud GM, Tran M¥, Villa-Sharma A¥, Huang M, Genereux JC. Bait correlation improves interactor identification by Tandem Mass Tag-Affinity Purification-Mass Spectrometry. J Prot Res 2020 Apr 3;19(4):1565-1573. doi: 10.1021/acs.jproteome.9b00825. Epub 2020 Mar 25. PubMed PMID: 32138514; eScholarship:https://escholarship.org/uc/item/3jr63127 * co-first author.  
  11. Plate L*, Rius B*, Nguyen B, Genereux JC, Kelly JW, Wiseman RL. Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein. Cell Chem Biol. 2019 Jul 18;26(7):913-925.e4. doi: 10.1016/j.chembiol.2019.04.001. Epub 2019 May 16. PubMed PMID: 31105062; PubMed Central PMCID: PMC6642020.
  12. Nguyen KK*, Thurmond S*, Hai R§, Genereux JC§. Protein profiling and pseudo-parallel reaction monitoring to monitor a fusion-associated conformational change in hemagglutinin. Anal Bioanal Chem. 2019 Jul;411(19):4987-4998. doi: 10.1007/s00216-019-01921-z. Epub 2019 Jun 28. PubMed PMID: 31254054.
  13. Wong MY, Chen K, Antonopoulos A, Kasper BT, Dewal MB, Taylor RJ, Whittaker CA, Hein PP, Dell A, Genereux JC, Haslam SM§, Mahal LK§, Shoulders MD§. XBP1s activation can globally remodel N-glycan structure distribution patterns. Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10089-E10098. doi: 10.1073/pnas.1805425115. Epub 2018 Oct 10. PubMed PMID: 30305426; PubMed Central PMCID: PMC6205500.
  14. Yang H, Mei L,Wang P, Genereux JC, Wang Y, Yi B, Au C,Danga L, Feng P. Photocatalytic degradation of norfloxacin on different TiO2−X polymorphs under visible light in water. RSC Adv. 2017. 7, 45721. doi:10.1039/C7RA09022F.
  15. Hulleman JD*, Genereux JC*, Nguyen A. Mapping wild-type and R345W fibulin-3 intracellular interactomes. Exp Eye Res. 2016 Dec; 153:165-169. doi: 10.1016/j.exer.2016.10.017. Epub 2016 Oct 21. PubMed PMID: 27777122; PubMed Central PMCID: PMC5433432. * co-first author
  16. Chen JJ*, Genereux JC*, Suh EH, Vartabedian VF, Rius B, Qu S, Dendle MTA, Kelly  JW, Wiseman RL. Endoplasmic Reticulum Proteostasis Influences the Oligomeric State of an Amyloidogenic Protein Secreted from Mammalian Cells. Cell Chem Biol. 2016 Oct 20;23(10):1282-1293. doi: 10.1016/j.chembiol.2016.09.001. Epub 2016 Oct 6. PubMed PMID: 27720586; PubMed Central PMCID: PMC5108364. * co-first author
  17. Plate L, Cooley CB, Chen JJ, Paxman RJ, Gallagher CM, Madoux F, Genereux JC, Dobbs W, Garza D, Spicer TP, Scampavia L, Brown SJ, Rosen H, Powers ET, Walter P, Hodder P, Wiseman RL, Kelly JW. Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation. Elife. 2016 Jul 20;5. pii: e15550. doi: 10.7554/eLife.15550. PubMed PMID: 27435961; PubMed Central PMCID: PMC4954754.
  18. Baranczak A, Liu Y, Connelly S, Du WG, Greiner ER, Genereux JC, Wiseman RL, Eisele YS, Bradbury NC, Dong J, Noodleman L, Sharpless KB, Wilson IA, Encalada SE, Kelly JW. A fluorogenic aryl fluorosulfate for intraorganellar transthyretin imaging in living cells and in Caenorhabditis elegans. J Am Chem Soc. 2015 Jun 17;137(23):7404-14. doi: 10.1021/jacs.5b03042. Epub 2015 Jun 8. PubMed PMID: 26051248; PubMed Central PMCID: PMC4472559.
  19. Genereux JC*, Qu S*, Zhou M, Ryno LM, Wang S, Shoulders MD, Kaufman RJ, Lasmézas CI, Kelly JW, Wiseman RL. Unfolded protein response-induced ERdj3 secretion links ER stress to extracellular proteostasis. EMBO J. 2015 Jan 2;34(1):4-19. doi: 10.15252/embj.201488896. Epub 2014 Oct 31. PubMed PMID: 25361606; PubMed Central PMCID: PMC4291477. * co-first author
  20. Chen JJ*, Genereux JC*, Qu S, Hulleman JD, Shoulders MD, Wiseman RL. ATF6 activation reduces the secretion and extracellular aggregation of destabilized variants of an amyloidogenic protein. Chem Biol. 2014 Nov 20;21(11):1564-74. doi: 10.1016/j.chembiol.2014.09.009. Epub 2014 Oct 23. PubMed PMID: 25444553; PubMed Central PMCID: PMC4254654. * co-first author
  21. Zhang X, Liu Y, Genereux JC, Nolan C, Singh M, Kelly JW. Heat-shock response transcriptional program enables high-yield and high-quality recombinant protein production in Escherichia coli. ACS Chem Biol. 2014 Sep 19;9(9):1945-9. doi: 10.1021/cb5004477. Epub 2014 Aug 2. PubMed PMID: 25051296; PubMed Central PMCID:  PMC4168666.
  22. Tan YL, Genereux JC, Pankow S, Aerts JM, Yates JR 3rd, Kelly JW. ERdj3 is an endoplasmic reticulum degradation factor for mutant glucocerebrosidase variants linked to Gaucher's disease. Chem Biol. 2014 Aug 14;21(8):967-76. doi: 10.1016/j.chembiol.2014.06.008. PubMed PMID: 25126989; PubMed Central PMCID: PMC4136546.
  23. Ryno LM, Genereux JC, Naito T, Morimoto RI, Powers ET, Shoulders MD, Wiseman RL. Characterizing the altered cellular proteome induced by the stress-independent activation of heat shock factor 1. ACS Chem Biol. 2014 Jun 20;9(6):1273-83. doi: 10.1021/cb500062n. Epub 2014 Apr 16. PubMed PMID: 24689980; PubMed Central PMCID: PMC4076015. 
  24. Liu Y, Tan YL, Zhang X, Bhabha G, Ekiert DC, Genereux JC, Cho Y, Kipnis Y, Bjelic S, Baker D, Kelly JW. Small molecule probes to quantify the functional fraction of a specific protein in a cell with minimal folding equilibrium shifts. Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):4449-54. doi: 10.1073/pnas.1323268111. Epub 2014 Mar 3. PubMed PMID: 24591605; PubMed Central PMCID: PMC3970509.
  25. Rainbolt TK, Atanassova N, Genereux JC, Wiseman RL. Stress-regulated translational attenuation adapts mitochondrial protein import through Tim17A degradation. Cell Metab. 2013 Dec 3;18(6):908-19. doi: 10.1016/j.cmet.2013.11.006. PubMed PMID: 24315374; PubMed Central PMCID: PMC3904643.
  26. Suh EH, Liu Y, Connelly S, Genereux JC, Wilson IA, Kelly JW. Stilbene vinyl sulfonamides as fluorogenic sensors of and traceless covalent kinetic stabilizers of transthyretin that prevent amyloidogenesis. J Am Chem Soc. 2013 Nov 27;135(47):17869-80. doi: 10.1021/ja408230k. Epub 2013 Nov 18. PubMed PMID: 24180271; PubMed Central PMCID: PMC3879133. 
  27. Leung A, Nah SK, Reid W, Ebata A, Koch CM, Monti S, Genereux JC, Wiseman RL, Wolozin B, Connors LH, Berk JL, Seldin DC, Mostoslavsky G, Kotton DN, Murphy GJ.  Induced pluripotent stem cell modeling of multisystemic, hereditary transthyretin amyloidosis. Stem Cell Reports. 2013 Oct 31;1(5):451-63. doi: 10.1016/j.stemcr.2013.10.003. eCollection 2013. PubMed PMID: 24286032; PubMed Central PMCID: PMC3841264.
  28. Shoulders MD, Ryno LM, Genereux JC, Moresco JJ, Tu PG, Wu C, Yates JR 3rd, Su AI, Kelly JW, Wiseman RL. Stress-independent activation of XBP1s and/or ATF6 reveals three functionally diverse ER proteostasis environments. Cell Rep. 2013 Apr 25;3(4):1279-92. doi: 10.1016/j.celrep.2013.03.024. Epub 2013 Apr 11. PubMed PMID: 23583182; PubMed Central PMCID: PMC3754422.
  29. Genereux JC, Wuerth SM¥, Barton JK. Single-step charge transport through DNA over long distances. J Am Chem Soc. 2011 Mar 23;133(11):3863-8. doi: 10.1021/ja107033v. Epub 2011 Feb 24. PubMed PMID: 21348520; PubMed Central PMCID: PMC3079569.
  30. Boal AK, Genereux JC, Sontz PA, Gralnick JA, Newman DK, Barton JK. Redox signaling between DNA repair proteins for efficient lesion detection. Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15237-42. doi: 10.1073/pnas.0908059106. Epub 2009 Aug 31. PubMed PMID: 19720997; PubMed Central PMCID: PMC2741234.
  31. Genereux JC, Augustyn KE, Davis ML¥, Shao F, Barton JK. Back-electron transfer suppresses the periodic length dependence of DNA-mediated charge transport across adenine tracts. J Am Chem Soc. 2008 Nov 12;130(45):15150-6. doi: 10.1021/ja8052738. Epub 2008 Oct 15. PubMed PMID: 18855390; PubMed Central PMCID: PMC2663386.
  32. Elias B, Genereux JC, Barton JK. Ping-pong electron transfer through DNA. Angew Chem Int Ed Engl. 2008;47(47):9067-70. doi: 10.1002/anie.200803556. PubMed PMID: 18925595; PubMed Central PMCID: PMC2748353.
  33. Augustyn KE, Genereux JC, Barton JK. Distance-independent DNA charge transport across an adenine tract. Angew Chem Int Ed Engl. 2007;46(30):5731-3. PubMed PMID: 17607671.
  34. Pasternack RF, Gibbs EJ, Sibley S, Woodard L, Hutchinson P, Genereux J, Kristian K. Formation kinetics of insulin-based amyloid gels and the effect of added metalloporphyrins. Biophys J. 2006 Feb 1;90(3):1033-42. Epub 2005 Nov 4. PubMed PMID: 16272434; PubMed Central PMCID: PMC1367090.
     


Reviews
 

  1. Genereux JC. Profiling Protein Targets of Cellular Toxicant Exposure. Mol Omics 2023. Accepted
  2. Lyu Z, Genereux JC. Measuring Protein Trafficking across Cellular Membranes. ChemPlusChem 2021. 86(10):1397-1415. doi: 10.1002/cplu.202100304. 
  3. Chen JJ, Genereux JC, Wiseman RL. Endoplasmic reticulum quality control and systemic amyloid disease: Impacting protein stability from the inside out. IUBMB Life. 2015 Jun;67(6):404-13. doi: 10.1002/iub.1386. Epub 2015 May 26. Review. PubMed PMID: 26018985; PubMed Central PMCID: PMC4485578.
  4. Genereux JC, Wiseman RL. Regulating extracellular proteostasis capacity through the unfolded protein response. Prion. 2015;9(1):10-21. doi: 10.1080/19336896.2015.1011887. PubMed PMID: 25946012; PubMed Central PMCID: PMC4601297
  5. Genereux JC, Barton JK. Mechanisms for DNA charge transport. Chem Rev. 2010 Mar 10;110(3):1642-62. doi: 10.1021/cr900228f. Review. PubMed PMID: 20214403; PubMed Central PMCID: PMC2879062
  6. Genereux JC, Boal AK, Barton JK. DNA-mediated charge transport in redox sensing and signaling. J Am Chem Soc. 2010 Jan 27;132(3):891-905. doi:  10.1021/ja907669c. PubMed PMID: 20047321; PubMed Central PMCID: PMC2902267.

Book Chapters
 

  1. Genereux JC. Mass spectrometric approaches for profiling protein folding and stability. Adv Prot Chem Struct Biol: Protein Misfolding. 2019, Volume 118, 111-144.

     

Commentary
 

  1. Castellanos JP, Genereux JC. Calreticulin surface presentation: a signal for natural killer cells to attack. Sig Transduct Target Ther 8, 289 (2023).
  2. Genereux JC, Wiseman RL. Chemically targeting the emergent properties of a chaperone complex. Chem Biol. 2011 Feb 25;18(2):144-5. doi: 10.1016/j.chembiol.2011.02.002. PubMed PMID: 21338912.
  3. Genereux JC, Barton JK. Molecular electronics: DNA charges ahead. Nat Chem. 2009 May;1(2):106-7. doi: 10.1038/nchem.188. PubMed PMID: 21378817.

 

 

 

 
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